Study of amino acid producing Micro organism from soil

Study of amino acid producing Micro organism from soil

Department of Biotechnology

Royal College,

Mira Road

year 2009-2010

Studies carried out at

Laboratory of Department of Biotechnology

Royal College, Mira Road

(Affiliated to Mumbai University)

Mumbai – Maharashtra – India

Submitted By

Mr. Nadim N. Dinani

Ms. Rujuta Vaidya & Ms. Pratiksha Rao

Students of Bsc.

Department of Biotechnology

Royal College, Mira Road

Abstract

Amino acid producers are of immense industrial importance as they are capable of producing amino acid such as L-glutamate, lysine, arginine, etc. These amino acids have numerous applications in food industries, pharma companies, etc.

Soil is considered to be a rich source of a variety of micro organisms. Hence it becomes important to isolate these organisms from soil micro flora.

This was achieved by use of screening procedures which included inoculation of soil sample in M9 agar broth for achievement of desired microbes. After isolation of enriched samples on M9 agar plates, colonies were confirmed for amino acid production followed by separation and identification of the amino acid using paper chromatography. Samples were also exposed to changes in conditions of temperature and pH to observe changes in growth pattern and characteristics. Those which tested positive were further grown and analyzed for determination of concentration of amino acid they could produce. The cultures producing amino acid were then subjected to UV radiation at different time intervals to introduce mutation and once again the yield was seen. Amino acid producers obtained can thus be used in industries to produce amino acid in large quantities.

Amino acid for synthetic applications serve as peptide hormones and growth factors, immunologic antigens, enzyme substrates, receptors and ligands, chemical drugs, bioactive peptides for research, drug discovery, pesticides, artificial sweetners, etc.